TRPA1 Promotes Cardiac Myofibroblast Transdifferentiation after Myocardial Infarction Injury via the Calcineurin-NFAT-DYRK1A Signaling Pathway

Figure 5

TRPA1 overexpression stimulates Ca²⁺ influx events in CFs. (a and b) Immunofluorescence staining (a) and quantification (b) of Fluo-4 in neonatal WT mouse primary CFs treated with or without TGF-β or transfected with Ad-TRPA1 (Ad) or si-TRPA1 (si) and treated 48 hr later with TGF-β (scale  μm). per group. (c and d) Representative traces (c) and frequencies (d) of Ca²⁺ oscillation ( cells) in single-cell Ca²⁺ imaging experiments. Ca²⁺ signals are presented as values, where is the Fluo-4 fluorescence value after treatment and is the value before treatment. per group. The error bars represent the and . The data are representative of the results of three or more independent experiments.

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