Counteraction of HCV-Induced Oxidative Stress Concurs to Establish Chronic Infection in Liver Cell Cultures

Figure 5

HCV induction by auranofin treatment. (a) Quantitative cytofluorimetric analysis of intracellular ROS production during the chronic phase of infection in cells treated or untreated with auranofin (Au) 200 nM and then stained with CellROX deep red. Left panel: results from one representative experiment out of three performed, are shown. Numbers represent the median fluorescence intensity. Right panel: of the results obtained from three independent experiments. In ordinate, median fluorescence intensity. vs. CTR uninfected cells. (b) RT-PCR analysis of HCV viral titer in the absence or presence of Au. vs. untreated infected cells. (c) Left panel: flow cytometry analysis after double staining of cells with annexin V-FITC/propidium iodide. Results from one representative experiment out of three performed, are shown. Right panel: percentage of apoptotic cells in uninfected (CTR) and HCV-infected Huh7.5 cells in the presence or absence of Au. Data are the of three independent experiments. vs. untreated infected cells.

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