UFL1 Alleviates Lipopolysaccharide-Induced Cell Damage and Inflammation via Regulation of the TLR4/NF-κB Pathway in Bovine Mammary Epithelial Cells

Figure 2

UFL1 protects the BMECs against LPS-induced inflammatory damage. (a) Effect of UFL1 siRNA on apoptosis in LPS-challenged BMECs. (b) Effect of UFL1 overexpression on apoptosis in LPS-challenged BMECs. (c) Effect of UFL1 on the protein expression of Bax and BCl-2 in BMECs. (d) Representative Western blots and quantitative evaluation of Bax and Bcl-2 in UFL1 siRNA-transfected BMECs stimulated with LPS. (e) Representative Western blots and quantitative evaluation of Bax and Bcl-2 in BMECs transfected with UFL1 overexpression plasmid stimulated with LPS. (f) Effect of UFL1 siRNA on activity of caspase-3 in LPS-challenged BMECs. (g) Effect of UFL1 overexpression on activity of caspase-3 in LPS-challenged BMECs. Data are presented as the of the mean (SEM) of three independent experiments. ; .

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