Research Article

UFL1 Alleviates Lipopolysaccharide-Induced Cell Damage and Inflammation via Regulation of the TLR4/NF-κB Pathway in Bovine Mammary Epithelial Cells

Figure 3

UFL1 mediates ER stress in LPS-stimulated BMECs. (a) Effect of UFL1 on the protein expression of CHOP, GRP78, and HSP70 in BMECs. (b) Representative Western blots and quantitative evaluation of CHOP, GRP78, and HSP70 in UFL1 siRNA-transfected BMECs stimulated with LPS. (c) Representative Western blots and quantitative evaluation of CHOP, GRP78, and HSP70 in BMECs transfected with UFL1 overexpression plasmid stimulated with LPS. (d) Immunofluorescent staining of BMECs with anti-GRP78 antibody in siRNA-treated cells stimulated with LPS. (e) Immunofluorescent staining of BMECs with anti-GRP78 antibody in UFL1 overexpression plasmid-treated cells stimulated with LPS. Data are presented as the of the mean (SEM) of three independent experiments. .
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