UFL1 Alleviates Lipopolysaccharide-Induced Cell Damage and Inflammation via Regulation of the TLR4/NF-κB Pathway in Bovine Mammary Epithelial Cells

Figure 4

UFL1 mediates autophagy in LPS-stimulated BMECs. (a) Effect of UFL1 on the protein expression of P62 and LC3-II in BMECs. (b) Representative Western blots and quantitative evaluation of P62 and LC3-II in UFL1 siRNA-transfected BMECs stimulated with LPS. (c) Representative Western blots and quantitative evaluation of P62 and LC3-II in BMECs transfected with UFL1 overexpression plasmid stimulated with LPS. (d) Immunofluorescent staining of BMECs with anti-LC3B antibody in siRNA-treated cells stimulated with LPS. (e) Immunofluorescent staining of BMECs with anti-LC3B antibody in UFL1 overexpression plasmid-treated cells stimulated with LPS. Data are presented as the of the mean (SEM) of three independent experiments. ; .

(a)

(b)

(c)

(d)

(e)