Oxidative Medicine and Cellular Longevity / 2019 / Article / Fig 2

Research Article

HDAC6 Inhibition Protects against OGDR-Induced Golgi Fragmentation and Apoptosis

Figure 2

Alteration of Golgi fragmentation and tubulin acetylation in cultured neurons after OGDR. The experiment was repeated independently for at least three times. (a) Immunofluorescent stain using antibodies against acetylated tubulin (green color) and Golgi marker TGN38 (red color) and counterstain with 4,6-diamidino-2-phenylindole (blue color) to show nuclei revealed ribbon-like GA adjacent to the nuclei in the control group. However, Golgi fragmentation increased greatly in cultured neurons after 2 h reperfusion following 4 h OGD. (b) Quantitation () of (a) from three independent experiments. The proportion of cultured neurons with fragmented GA increased after 2 h reperfusion following 4 h OGD exposure. (c) Quantitation () of (a) from three independent experiments. Tubulin acetylation was significantly decreased after 2 h reperfusion following 4 h OGD in cultured neurons. The western blot data also showed that OGDR insult decreased tubulin acetylation in cultured neurons. and compared to normal conditions. .
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