HDAC6 Inhibition Protects against OGDR-Induced Golgi Fragmentation and Apoptosis

Figure 4

Effects of GBF1 overexpression on GA morphology and apoptosis in N2a cells exposed to OGDR. N2a cells were transfected with different plasmids (pEGFP-N1 and pEGFP-GBF1). After transfection for 36 h, cells were treated with 4 h OGD plus 4 h reperfusion. The experiment was repeated independently for at least three times. (a) Digital photomicrograph under fluorescent illumination showing the morphology of GA which was detected using TGN38 staining. GA displayed typical ribbon-like structures adjacent to the nuclei in N2a cells transfected with pEGFP-N1 without OGDR exposure. Fragmented GA was evident in N2a cells transfected with pEGFP-N1 exposed to 4 h OGD plus 4 h reperfusion insult. Transfection with pEGFP-GBF1 significantly attenuated OGDR-induced fragmentation of GA. (b) Quantitation () of (a) from three independent experiments. (c) Overexpression of GBF1 in vitro reduced OGDR-induced apoptosis. Values are expressed as . compared to control. compared to control. .

(a)

(b)

(c)