Triptolide Inhibits Preformed Fibril-Induced Microglial Activation by Targeting the MicroRNA155-5p/SHIP1 Pathway

Figure 2

PFFs activated primary microglia by binding to TLR1/2 or TLR4. Primary microglia were pretreated with Cu-CPT22, resatorvid, or E6446 for 12 h and then treated with PFFs for further 24 h. Levels of TNFα (a) and IL-1β (b) were measured by ELISA assay. (c-f) Expressions of IRAK1, TRAF6, p-IKKα/β/IKKα/β ratio, and p-NF-κB/NF-κB ratio were measured by Western blot. (g) Translocations of NF-κB were monitored by immunofluorescence staining. Scale . The values were expressed as . .