Triptolide Inhibits Preformed Fibril-Induced Microglial Activation by Targeting the MicroRNA155-5p/SHIP1 Pathway

Figure 6

SHIP1 was involved in the regulation of T10 on NF-κB activity. The mRNA (a) and protein (b) levels of SHIP1 in primary microglia transfected with SHIP1 siRNA. (c-f) Ratios of p-PI3K/PI3K, p-Akt/t-Akt, p-IKKα/β/IKKα/β, and p-NF-κB/NF-κB were measured by Western blot in SHIP1-silenced primary microglia. (g) SHIP1 inhibitor, 3AC, abolished the inhibition of T10 on the ratio of p-NF-κB/NF-κB. (h-i) Levels of TNFα and IL-1β. (j) Levels of miRNA155-5p. The values were expressed as . and .