Methane-Rich Saline Counteracts Cholestasis-Induced Liver Damage via Regulating the TLR4/NF-κB/NLRP3 Inflammasome Pathway

Figure 6

Methane-rich saline reduced pyroptosis via inhibiting inflammasome signaling pathway activation. MRS treatment in rats was achieved by intraperitoneal administration (10 mL/kg) for 7 days after BDL. (a) Immunoblot assays of NLRP3, caspase-1, and IL-1β. Relative densities of (b–d) NLRP3, caspase-1, and IL-1β. (e) Immunohistochemical staining of NLRP3 and caspase-1 (scale bars: 20 μm). The percentage of (f, g) NLRP3 and caspase-1. (h) IL-1β serum levels (. Data are shown as the mean ± SD. versus the sham control group; ^# versus the sham control group; ^€ versus the MRS control group; ^$ versus the MRS control group; ^& versus the BDL+NS group; ^γ versus the BDL+NS group).

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)