miR-146a-5p Mediates Intermittent Hypoxia-Induced Injury in H9c2 Cells by Targeting XIAP

Figure 4

XIAP is a target gene of miR-146a-5p, and XIAP expression could be negatively regulated by miR-146a-5p in H9c2 cells. (a) The putative binding site for miR-146a-5p in the 3-UTR of XIAP mRNA. (b) Luciferase reporter assay. Cells were cotransfected with wild-type or mutant XIAP 3-UTR reporters and miR-146a-5p mimics or corresponding control. (c–e) H9c2 cells were transfected with miR-146a-5p mimics or corresponding control. mRNA and protein expressions of XIAP were analyzed by western blot analysis. . (Data are presented as the of three independent experiments. , , and ).

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