miR-146a-5p Mediates Intermittent Hypoxia-Induced Injury in H9c2 Cells by Targeting XIAP

Figure 5

The effects of miR-146a-5p inhibition in H9c2 cells under IH condition are reversed by XIAP knockdown. miR-146a-5p inhibitor, corresponding scrambled control, and small interfering RNA targeting XIAP (si-XIAP) were transfected into H9c2 cells. Cells without transfection were acted as control. (a–f) Cell apoptosis by flow cytometry analysis. (g) Cell viability by a Cell Counting Kit-8. (h, i) Western blotting assays for XIAP, Bcl-2, Bax, and Caspase-3 protein expressions. Relative protein levels are presented as the average expressions normalized to actin. IH: intermittent hypoxia; . (Data are presented as the of three independent experiments. , , and ).

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