Research Article
T Cell-Derived IL-17A Induces Vascular Dysfunction via Perivascular Fibrosis Formation and Dysregulation of ⋅NO/cGMP Signaling
Figure 5
T cell-specific IL-17A overexpression results in a downregulation of sGC/VASP signaling in the aorta, with a concomitant upregulation of Vcam1 and Mmp2 mRNA. (a, b) Western blot of aortic protein (a) and PVAT protein (b) of CD4-IL-17Aind/+ and control mice of p-eNOS (S1177), eNOS, PYK2, sGCα1, sGCβ1, pVASP (S239), and GCH-1. a1, b1: original Western blot; a2, b2: quantification of the signal, aorta: (partially, 3-4 aortas were pooled per sample), Mann–Whitney -test or unpaired Student’s -test. PVAT: , Student’s unpaired -test. (c) Quantitative real-time PCR of aortic mRNA of Vcam1, Cybb, Nos3, Mmp2, Mmp9, and Tgfb, , Mann–Whitney -test or unpaired Student’s -test. Data are shown as .
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