Prooxidative Activity of Celastrol Induces Apoptosis, DNA Damage, and Cell Cycle Arrest in Drug-Resistant Human Colon Cancer Cells

Figure 6

Flow cytometric analysis of early and late apoptosis in LOVO/DX cell cultures incubated with celastrol or coincubated with celastrol and NAC. Representative cytograms are shown. FSC = forward light scatter, SSC = side light scatter, FL1 = Annexin V-FITC, FL3 = PI, Q1 = necrotic cells (Annexin V-FITC^- and PI⁺), Q2 = late apoptotic cells (Annexin V-FITC⁺ and PI⁺), Q3 = live cells (Annexin V-FITC^- and PI^-), Q4 = early apoptotic cells (Annexin V-FITC⁺ and PI^-).