Prooxidative Activity of Celastrol Induces Apoptosis, DNA Damage, and Cell Cycle Arrest in Drug-Resistant Human Colon Cancer Cells
Representative histograms of flow cytometric analysis of DNA damages (γ-H2AX+ cells) and cell cycle phases in LOVO/DX cells after treatment with celastrol. (a) Detection of γ-H2AX+ cells using phospho-histone H2A.X (Ser139) monoclonal antibody (CR55T33) Alexa Fluor 488. SSC = side light scatter. (b) Bivariant cell cycle/DAPI-combined γ-H2AX analysis.
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