Prooxidative Activity of Celastrol Induces Apoptosis, DNA Damage, and Cell Cycle Arrest in Drug-Resistant Human Colon Cancer Cells

<div>Representative histograms of flow cytometric analysis of DNA damages (<i>γ</i>-H2AX<sup>+</sup> cells) and cell cycle phases in LOVO/DX cells after treatment with celastrol. (a) Detection of <i>γ</i>-H2AX<sup>+</sup> cells using phospho-histone H2A.X (Ser139) monoclonal antibody (CR55T33) Alexa Fluor 488. SSC = side light scatter. (b) Bivariant cell cycle/DAPI-combined <i>γ</i>-H2AX analysis.</div>

Oxidative Medicine and Cellular Longevity

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Figure 8

Figure 8: Prooxidative Activity of Celastrol Induces Apoptosis, DNA Damage, and Cell Cycle Arrest in Drug-Resistant Human Colon Cancer Cells 