Increased Oxidative Stress Induced by Rubus Bioactive Compounds Induce Apoptotic Cell Death in Human Breast Cancer Cells

Figure 9

Immunofluorescence: caspase 9, p53, and Bax. (a) Control cells showing very few caspase 9-positive cells; (b) C1 4.69 μg/mL treated showing higher caspase 9-positive cells; (c) C2 8.36 μg/mL treated showing medium caspase 9-positive cells. (d) Control showing very few nuclear staining for active p53; (e) C1 4.69 μg/mL treated showing strong nuclear staining for active p53; (f) C2 8.36 μg/mL treated showing moderate nuclear staining for active p53. (g) Control showing weak staining for active Bax; (h) C1 4.69 μg/mL treated showing strong cytoplasmic staining for active Bax; (i) C2 8.36 μg/mL treated showing moderate cytoplasmic staining for active Bax. C1-treated cells showed increased expression of caspase 9, p53, and Bax proteins than C2-treated cells (×20, original magnification; arrow indicates the presence of proteins; nuclear stain: DAPI (blue color); p53 and Bax protein stain: FITC (green color)).

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