Research Article
In Vitro Anti-Inflammatory Effect of Salvia sagittata Ethanolic Extract on Primary Cultures of Porcine Aortic Endothelial Cells
Table 1
Primer sequences used for quantitative real-time polymerase chain reaction analysis.
| | Gene | Sequence (5-3) | PCR product (bp) | GenBank accession number | Reference |
| | HO-1 | For: CGCTCCCGAATGAACAC
Rev: GCTCCTGCACCTCCTC | 112 | NM_001004027 | Bernardini et al. [31] |
| | IL-8 | For: AGGACCAGAGCCAGGAAGAGAC
Rev: TGGAAAGGTGTGGAATGCGTATTTATG | 203 | AB057440.1 | Present study |
| | IL-6 | For: AGCAAGGAGGTACTGGCAGAAAACAAC
Rev: GTGGTGATTCTCATCAAGCAGGTCTCC | 110 | AF518322.1 | Zannoni et al. [32] |
| | GAPDH | For: ACATGGCCTCCAAGGAGTAAGA
Rev: GATCGAGTTGGGGCTGTGACT
Probe: HEX-CCACCAACCCCAGCAAGAGCACGC-BHQ1 | 106 | NM_001206359 | Duvigneau et al. [33] |
| | HPRT | For: ATCATTATGCCGAGGATTTGGAAA
Rev: TGGCCTCCCATCTCTTTCATC
Probe: Tx-Red-CGAGCAAGCCGTTCAGTCCTGTCC-BQ2 | 102 | NM_001032376 | Present study |
| | β-ACT | For: CTCGATCATGAAGTGCGACGT
Rev: GTGATCTCCTTCTGCATCCTGTC
Probe: FAM-ATCAGGAAGGACCTCTACGCCAACACGG-BHQ1 | 114 | KU672525.1 | Duvigneau et al. [33] |
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