Syk and Hrs Regulate TLR3-Mediated Antiviral Response in Murine Astrocytes

Figure 3

NF-κB nuclear translocation is downregulated in poly(I:C)-treated astrocytes with silenced Syk and Hrs. C8-D1A cells were untreated or treated with poly(I:C) or poly(I:C)/LyoVec for 5 min, 8 min, 12 min, 15 min, 30 min, and 60 min. In advance to the stimulation, astrocytes were not transfected (a) or transfected with siRNA pools for TLR3 (b), Syk (c), and Hrs (d). Following poly(I:C) or poly(I:C)/LyoVec treatment, cytoplasmic and nuclear extracts were immunoblotted with anti-NF-κB p65, -IRF3, -IRF7, -GAPDH, and -PARP antibodies. (e) TLR3 and Hrs silencing efficiency was visualized by immunoblotting with anti-TLR3 and -Hrs antibodies.

(a)

(b)

(c)

(d)

(e)