Syk and Hrs Regulate TLR3-Mediated Antiviral Response in Murine Astrocytes

Figure 4

Knockdown of Syk and Hrs expression by siRNA upregulates poly(I:C)-induced IFN-β, IL-6, and CXCL-8 production. C8-D1A cells were transfected with control siRNA-A or siRNA pools for TLR3, Syk, Hrs, and STAM. Following the transfection, astrocytes were treated with poly(I:C) (10 μg/ml) for 24 h. IFN-β (a), IL-6 (b), and CXCL-8 (c) were measured in culture supernatants by ELISA. Because Syk transfection lasted 48 h, in each experiment, supernatants from untreated (not treated (Syk)), poly(I:C)-treated (poly(I:C) (Syk)), and poly(I:C)-treated cells with silenced Syk (siRNA Syk) were tested in the group independent from cells with silenced TLR3, Hrs, and STAM, where transfection lasted 72 h. (d) STAM silencing efficiency was visualized by immunoblotting with anti-STAM antibodies. Data was obtained from three (IFN-β, CXCL-8) or five (IL-6) independent experiments and presented as . and .

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