Syk and Hrs Regulate TLR3-Mediated Antiviral Response in Murine Astrocytes

Figure 5

TLR3 of murine astrocytes is cleaved upon stimulation of cells with poly(I:C). Representative western blots of TLR3 expression in C8D1A cells treated with various concentrations of poly(I:C) (0, 0.1, 1, 2, 5, and 10 μg/ml) (a) or poly(I:C)-LyoVec (0, 0.1, 1, 2, and 5 μg/ml) (b) and lysed 24 h after stimulation. TLR3 expression was also analyzed in cells treated with poly(I:C) at concentration 10 μg/ml (c), or with poly(I:C)-LyoVecat concentration 1 μg/ml (d), and lysed at various times of stimulation (0, 30 min, 1 h, 2 h, 4 h, 8 h, and 24 h). TLR3 FL: full-length TLR3; TLR3 N: cleaved N-terminal TLR3 form; GAPDH: protein loading control. Densitometry analysis of TLR3 forms was performed in cells treated with indicated poly(I:C) concentrations for 24 h (a), indicated poly(I:C)/LyoVecconcentrations for 24 h (b), 10 μg/ml poly(I:C) for indicated time points (c), or 1 μg/ml poly(I:C)/LyoVec for indicated time points (d). The density level of each protein was normalized to GAPDH. Data was obtained from three independent experiments and presented as . and .

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