Syk and Hrs Regulate TLR3-Mediated Antiviral Response in Murine Astrocytes

Figure 6

Stimulation of murine astrocytes with poly(I:C) leads to the time-dependent increase in Syk and Hrs expression, while the expression of STAM does not significantly change after stimulation of cells with the TLR3 ligand. Representative western blots of Hrs, Syk, and STAM expression in C8D1A cells treated with various concentrations of poly(I:C) (0, 0.1, 1, 2, 5, and 10 μg/ml) (a), or poly(I:C)-LyoVec (0, 0.1, 1, 2, and 5 μg/ml) (b), and lysed 24 h after stimulation. Hrs, Syk, and STAM expression was also analyzed in cells treated with poly(I:C) at concentration 10 μg/ml (c), or with poly(I:C)-LyoVecat concentration 1 μg/ml (d), and lysed at various times of stimulation (0, 30 min, 1 h, 2 h, 4 h, 8 h, and 24 h). GAPDH was used for evaluating protein loading control. Densitometry analysis of Hrs, Syk, and STAM was performed in cells treated with indicated poly(I:C) concentrations for 24 h (a), indicated poly(I:C)/LyoVecconcentrations for 24 h (b), 10 μg/ml poly(I:C) for indicated time points (c), or 1 μg/ml poly(I:C)/LyoVec for indicated time points (d). The density level of each protein was normalized to GAPDH. Data was obtained from three independent experiments and presented as . and .

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