Syk and Hrs Regulate TLR3-Mediated Antiviral Response in Murine Astrocytes

Table 1

Primary antibodies used in the western blot assay.

Antibody	Clone/ID	Isotype	Source	Concentration
TLR3	PA5-23105^a	Polyclonal rabbit	Thermo Fisher Scientific	2 μg/ml
TLR3	MA5-16184^a	Monoclonal mouse	Thermo Fisher Scientific	2 μg/ml
TLR3	TLR3.7^b	Monoclonal mouse	OriGene Technologies GmbH	2 μg/ml
Hrs	15087^a	Monoclonal rabbit	CST	1 : 1000
Hrs	M-79^a	Polyclonal rabbit	Santa Cruz Biotechnology	1 : 200
Hrs	C-7^a	Monoclonal mouse	Santa Cruz Biotechnology	1 : 200
STAM	13053^a	Polyclonal rabbit	CST	1 : 200
STAM	H-175^a	Polyclonal rabbit	Santa Cruz Biotechnology	1 : 100
STAM	B-2^a	Monoclonal mouse	Santa Cruz Biotechnology	1 : 200
Syk	N-19^a	Polyclonal rabbit	Santa Cruz Biotechnology	1 : 200
Syk	4D10^a	Monoclonal mouse	Santa Cruz Biotechnology	1 : 200
PDI	RL77^b	Monoclonal mouse	Thermo Fisher Scientific	1 : 100
Phospho-Syk	I120-722^b	Monoclonal mouse	Becton Dickinson Biosciences	1 : 500
Phosphotyrosine	4G10^a	Monoclonal mouse	Merck	1 : 1000
Phosphotyrosine	61-5800^a	Polyclonal rabbit	Thermo Fisher Scientific	1 : 1000
IRF3	4302^a	Monoclonal rabbit	CST	1 : 1000
NF-ĸB p65	8242^a	Monoclonal rabbit	CST	1 : 1000
IRF7	PA1-12810^a	Polyclonal rabbit	Thermo Fisher Scientific	2 μg/mL
PARP	9532^a	Monoclonal rabbit	CST	1 : 1000
Ubiquitin	P4D1^a	Monoclonal mouse	Santa Cruz Biotechnology	1 : 200
Ubiquitin	3933^a	Polyclonal rabbit	CST	1 : 1000
GAPDH	MA5-15738^a	Monoclonal mouse	Thermo Fisher Scientific	1 : 1000

^aManufacturer’s antibody identification. ^bClone