Cold Physical Plasma Modulates p53 and Mitogen-Activated Protein Kinase Signaling in Keratinocytes

Figure 3

Cold plasma alters phosphorylation level of p53 in a treatment and incubation time-dependent manner. The upper graphs showed the treatment time-dependent activation of p53. Displayed are relative p53 phosphorylation levels of residues Ser15 (a) and Ser37 (b) normalized to total p53 and β-actin expression. Bottom graphs displayed the time courses of relative phosphorylation after longest plasma treatment of relative p53-Ser15 (c) and Ser37 phosphorylation (d). Untreated samples were included as negative control (ctrl). Data are presented as mean + S.D. of two independent experiments. The x-axis represents treatment time (a, b) or incubation after plasma treatment (c, d). Statistical comparison was done using one-way ANOVA with Dunnett corrections for multiple comparison to untreated control, normalized control (, , ).

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