Research Article

Cold Physical Plasma Modulates p53 and Mitogen-Activated Protein Kinase Signaling in Keratinocytes

Figure 6

Plasma-induced activation of MAP kinase signaling in HaCaT keratinocytes. Displayed are relative phosphorylation levels of Erk (p-T202/Y204, a), Jnk (p-T183/Y185 (b)), and p38 (p-T180/Y182, (c) kinases after different treatment times. Lower panels showed the results for time course of relative phosphorylation levels after 180 s of plasma treatment for Erk (d), Jnk (e), and p38 (f). Each expression was normalized to total protein expression. Representative blots are shown. Data are presented as mean + S.D. of two analyses. The x-axis represents treatment time (a–c) or incubation after plasma treatment (d–f). Statistical analysis was done using one-way ANOVA with Dunnett corrections for multiple comparisons to untreated, normalized control (, , ).
(a)
(b)
(c)
(d)
(e)
(f)