Cold Physical Plasma Modulates p53 and Mitogen-Activated Protein Kinase Signaling in Keratinocytes

Figure 7

Cold plasma-induced impact on major p53 downstream targets. The total amount and the phosphorylated form of the stress-related protein HSP27 was significantly enhanced after plasma treatment (a). Agarose gel showing semiquantitative PCR of BAX, BBC3, GADD45, and CDKN1A after 1 to 24 h posttreatment of HaCaT cells with 60 s plasma (b). mRNA copy numbers of all four targets were measured 6 h after plasma treatment by qPCR and normalized to the relative gene expression (ΔΔCT values on a log2 scale) (c). Puma (protein of BBC3), Gadd45 (d), and p21 (e) expression was significantly enhanced after plasma exposure (180 s). p21 (protein of CDKN1A) increased until 6 hours after plasma treatment, declining afterwards (f). Representative blots are shown. The x-axis represents treatment time (a, c, d, and e) or incubation after plasma treatment (b, f). Data are presented as mean + S.D. of two (c, d) or three (b) analyses. Statistical analysis was done using one-way ANOVA with Dunnett corrections for multiple comparisons to untreated, normalized control (, , ).

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