Nrf2 Overexpression for the Protective Effect of Skin-Derived Precursors against UV-Induced Damage: Evidence from a Three-Dimensional Skin Model

Figure 7

PI3K/Akt inhibitor LY294002 attenuated the photoprotection of nrf2-over-SKPs in 3D skin equivalent. (a) The protein levels of Nrf2, HO-1, p-PI3K, and p-Akt in 3D skin equivalents by Western blot analysis; (b) quantitative analysis of apoptotic cells in 3D skin equivalents by TUNEL staining. and ; (c) ELISA assay for ROS expression in the supernatants of 3D skin equivalents. and ; (d) ELISA assay for SOD and GPX expressions in the supernatants of 3D skin equivalents. , , , and .

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