Antiaging, Stress Resistance, and Neuroprotective Efficacies of Cleistocalyx nervosum var. paniala Fruit Extracts Using Caenorhabditis elegans Model

Figure 4

C. nervosum extracts are dependent and independent of DAF-16-mediated pathway. (a) qPCR analysis of candidate genes of DAF-16-mediated pathway. Wild-type nematodes treated with CMK-P and LMK-P extracts at 20 and 30 μg/ml showed significant () upregulation in the expression of daf-16 at selective doses and corresponding significant () downregulation of daf-2, age-1, and utx-1. (b) CMK-P at 10, 20, 30, and 40 μg/ml could extend the maximum lifespan of daf-16 mutants. (c) Graph showing significant increase in the average maximum survival days of daf-16 mutant nematodes treated with 10, 20, 30, and 40 μg/ml of CMK-P extracts. (d) Graph showing significant increase in the average median survival days of daf-16 mutant nematodes treated with 10, 20, 30, and 40 μg/ml of CMK-P extracts. (e) LMK-P at 10, 20, 30, and 40 μg/ml could extend the maximum lifespan of daf-16 mutants. (f) Graph showing significant increase in the average maximum survival days of daf-16 mutant nematodes treated with 10, 20, 30, and 40 μg/ml of LMK-P extracts. (g) Graph showing significant increase in the average median survival days of daf-16 mutant nematodes treated with 10, 20, 30, and 40 μg/ml of LMK-P extracts.

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