Oxidative Medicine and Cellular Longevity / 2019 / Article / Fig 2

Research Article

Abnormal Ribosome Biogenesis Partly Induced p53-Dependent Aortic Medial Smooth Muscle Cell Apoptosis and Oxidative Stress

Figure 2

Overexpression of BOP1 attenuated HASMC apoptosis under serum-free and hypoxia condition. (a) The expression of BOP1 in HASMCs was detected after it had been infected with Ad-BOP1 or Ad-GFP for 24 h (left panel). Statistical analysis is shown (right panel). (b) HASMCs were infected with Ad-BOP1 or Ad-GFP for 24 h. The cells were suspended and reseeded in 96-well plates. CCK-8 assays were performed to assess the influence of BOP1 on HASMC proliferative ability. The growth curve is shown. (c) After being infected with Ad-BOP1 or Ad-GFP for 24 h, HASMCs were administrated in serum-free and hypoxia condition for the time shown. Apoptosis was detected by Annexin V-APC/7-AAD staining and flow cytometry followed. The representative images are shown. (d) The statistical analysis of apoptosis rate is shown. (e) Western blotting was performed to detect the BOP1, p53, α-SMA, activated caspase 3, and MLC expression. The representative image is shown (left panel). The statistical analysis is shown (right panel). Data are representative of three independent experiments and presented as . , , and determined by one-way ANOVA.

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