Methane Alleviates Acetaminophen-Induced Liver Injury by Inhibiting Inflammation, Oxidative Stress, Endoplasmic Reticulum Stress, and Apoptosis through the Nrf2/HO-1/NQO1 Signaling Pathway

Figure 4

MRS inhibited ERS-induced apoptosis after APAP challenge. (a) TUNEL fluorescence staining (green), nuclear counterstaining (blue), and merging of both channels in the representative liver sections (200x); (b) percentage of TUNEL-positive cells; (c, d) western blot results of GRP78, ATF4, CHOP, Bcl-2, and Bax expressions in the liver; (e) IHC staining (200x) showing Bax and Bcl-2 expressions in the liver; and (f) relative IHC staining scores (, , , and ).

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