Tetramethylpyrazine Prevents Contrast-Induced Nephropathy via Modulating Tubular Cell Mitophagy and Suppressing Mitochondrial Fragmentation, CCL2/CCR2-Mediated Inflammation, and Intestinal Injury

Figure 4

TMP restored mitochondrial dynamic-related protein expression in CIN rats. (a) Drp1 expression was assessed by immunohistochemistry (IHC) in the kidneys of Con (A1), CIN (A2), CIN+NAC (A3), and CIN+TMP (A4) rats (original magnification, ×200). (b) Evaluation of Mfn2 expression in the kidneys of Con (B1), CIN (B2), CIN+NAC (B3), and CIN+TMP (B4) rats by IHC (original magnification, ×200). Semiquantitative analysis of IHC staining for Drp1 (c) and Mfn2 (d). (e) Western blotting analyses of Drp1 and Mfn2 in the kidneys of rats. Relative densitometry analysis of the ratio of Drp1 (f) or Mfn2 (g) to GAPDH. Figures are representative of 5-8 rats from each group. Data are represented as (). versus Con and ^## versus CIN.

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