HIF1α-Induced Glycolysis in Macrophage Is Essential for the Protective Effect of Ouabain during Endotoxemia

Figure 5

HIF1α is required for regulating the anti-inflammatory effect of ouabain. (a) PMs were examined for HIF1α protein levels (left) and gene transcripts (right) at the indicated time points using Western blot and real-time PCR. (b) Cells were stimulated with LPS (100 ng/ml) for 6–8 h followed by DMOG (0.5 mmol/l) stimulation for 3 h. TNF-α and IL-6 were determined by ELISA. (c) RAW264.7 cell lines transfected with plasmid-transfected HIF1α specific vector and blank vector. 24 h later, cells were treated with 2 mmol/l ouabain for 0.5 h and then stimulated with LPS for 6 h. Supernatants were assayed for TNF-α and IL-6 levels by ELISA. . , ANOVA.

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