Synthetic Imine Resveratrol Analog 2-Methoxyl-3,6-Dihydroxyl-IRA Ameliorates Colitis by Activating Protective Nrf2 Pathway and Inhibiting NLRP3 Expression
C33 inhibits the expression of nitrotyrosine in colitis mice. IHC staining of nitrotyrosine in colon sections from mice in Figure 3(a). WT mice were treated with C33 (200 mg/kg i.g.), RSV (200 mg/kg i.g.), or vehicle (10% ethanol) daily. Two days later, while the mice continued with the daily RSV or C33, they were also given normal drinking water or water containing 2.5% DSS for 7 days. After cessation of DSS exposure and RSV or C33 treatment, mice were given water for further three days. Water (a), mice on normal drinking water without any treatment. +DSS (b–d), mice on drinking water containing 2.5% DSS. Scale bars, 100 μm; original magnification ×200. (e) Semiquantitative results. The WT mice on drinking water without any treatment (control) was set at 1. Values are ().,, difference between the colitis WT mice with different treatments. ##, difference between the WT mice on normal drinking water without any treatment and the colitis WT mice with different treatments.