Synthetic Imine Resveratrol Analog 2-Methoxyl-3,6-Dihydroxyl-IRA Ameliorates Colitis by Activating Protective Nrf2 Pathway and Inhibiting NLRP3 Expression

Figure 6

C33 inhibits the expression of nitrotyrosine in colitis mice. IHC staining of nitrotyrosine in colon sections from mice in Figure 3(a). WT mice were treated with C33 (200 mg/kg i.g.), RSV (200 mg/kg i.g.), or vehicle (10% ethanol) daily. Two days later, while the mice continued with the daily RSV or C33, they were also given normal drinking water or water containing 2.5% DSS for 7 days. After cessation of DSS exposure and RSV or C33 treatment, mice were given water for further three days. Water (a), mice on normal drinking water without any treatment. +DSS (b–d), mice on drinking water containing 2.5% DSS. Scale bars, 100 μm; original magnification ×200. (e) Semiquantitative results. The WT mice on drinking water without any treatment (control) was set at 1. Values are (). , , difference between the colitis WT mice with different treatments. ^##, difference between the WT mice on normal drinking water without any treatment and the colitis WT mice with different treatments.