MLN4924 Exerts a Neuroprotective Effect against Oxidative Stress via Sirt1 in Spinal Cord Ischemia-Reperfusion Injury
MLN4924 protects against H2O2-induced oxidative stress and cell death in SH-SY5Y cells via Sirt1. Cell proliferation in response to different doses of either (a) H2O2 or (b) MLN4924 was detected with a cell counting kit-8 (CCK8) assay. (c) SOD activity and (d) MDA concentration in SH-SY5Y cells treated with or without H2O2 and MLN4924/EX527. (e, f) FACS analysis of apoptosis in SH-SY5Y cells treated with or without H2O2 and MLN4924/EX527. H2O2: 200 μM; MLN4924: 110 nM; Ex527: 1 μM. ,,, and . values were analyzed by one-way analysis of variance (ANOVA).
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