Presenilin 1 Regulates [Ca²⁺]i and Mitochondria/ER Interaction in Cultured Rat Hippocampal Neurons

Figure 8

mPS1-transfected neurons are more sensitive to oxidative stress than controls. Cells were transfected with eBFP and PS1 or mPS1 and treated with paraquat (20 μM) for 24 h in the incubator. For each group, the entire glass was imaged on confocal microscope using tail mode (see Section 2 for details). All transfected cells in the glass were counted using squares and the number obtained was averaged for each glass. : 8 glasses for each group. A marked reduction in the mPS1-transfected neurons per glass was evident ().

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