Obeticholic Acid Protects against Gestational Cholestasis-Induced Fetal Intrauterine Growth Restriction in Mice

Figure 5

OCA pretreatment alleviated oxidative stress and protein nitration during E2-induced cholestasis. All pregnant mice except controls were s.c. injected with E2 (0.625 mg/kg) once daily from GD13 to GD17. In the OCA+E2 groups, pregnant mice were administered with OCA (5 mg/kg) by gavage once daily from GD12 to GD17. All dams were sacrificed on GD18. (a) Placental GSH level ( for each group); (b) placental MDA content ( for each group). (c) Fetal liver GSH level ( for each group); (d) fetal liver MDA content ( for each group). (e, f) Placental 3-NT residues were analyzed using immunoblot. (e) Representative gels were shown. (f) 3-NT/β-actin ( for each group). (g, h) Placental 3-NT residues were analyzed by IHC. (g) Representative photomicrographs were shown. Original magnification: ×400. Placental 3-NT residues were mainly distributed in mononuclear sinusoidal TGCs of the labyrinth zone (arrow). (h) The percentages of 3-NT⁺ cells were evaluated among different groups ( for each group). , .

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