Oxidative Stress Produced by Hyperthyroidism Status Induces the Antioxidant Enzyme Transcription through the Activation of the Nrf-2 Factor in Lymphoid Tissues of Balb/c Mice

Figure 2

Modulation of the levels of reactive oxygen species. Lymphocyte cells of euthyroid and hyperthyroid mice were incubated with 10 μM DCFH-DA for 30 min at 37°C, and the fluorescence intensity of the oxidized probe was measured by a flow cytometer at 488 nm. The histograms shown are representative of 8 independent experiments performed in duplicate. The average values of mean fluorescence intensity are indicated in the table. differs significantly from the euthyroid mice with .