Oxidative Stress Produced by Hyperthyroidism Status Induces the Antioxidant Enzyme Transcription through the Activation of the Nrf-2 Factor in Lymphoid Tissues of Balb/c Mice

Figure 3

Genomic and protein expression of antioxidant enzymes. (a) The genomic expression of CAT, GPX-1, and SOD-1 was evaluated by conventional PCR. The amplified products were subjected to agarose gel electrophoresis and stained with ethidium bromide. Representative gels from 4 independent experiments performed in duplicate are shown. The bar graph shows the densitometric analysis of each band relativized to β-microglobulin used as housekeeping. The smooth bars correspond to lymph node samples, and stripe bars correspond to the spleen samples. differs significantly from euthyroid controls with . (b) The genomic expression of the antioxidant enzymes was quantified by real-time PCR (bar graphs). differs significantly from euthyroid controls with or differs significantly from euthyroid controls with . (c) The protein expression of CAT (60 kDa), GPX-1 (22 kDa), and SOD (32 kDa) was determined by western blot assays from cell extracts obtained from lymph nodes and spleens of euthyroid and hyperthyroid mice. Representative immunoblots from 4 independent experiments are shown. The bar graph shows the densitometric analysis of each band relativized to β-tubulin used as housekeeping. differs significantly from the lymphoid cells of euthyroid mice with .

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