Nrf2 Activation Protects Mouse Beta Cells from Glucolipotoxicity by Restoring Mitochondrial Function and Physiological Redox Balance

Figure 5

Oltipraz and DMF alter Nrf2 distribution in islet cells stressed by glucolipotoxicity. Cells were treated by Nrf2 activators and cultured in glucolipotoxic medium as described for Figure 4. (a) Representative images out of 3-5 mouse preparations are shown (left: anti-Nrf2 antibody (green), middle: DAPI staining (blue), and right: overlay). Addition of oltipraz (10 μmol/L) or DMF (50 μmol/L) during glucolipotoxic culture increased nuclear Nrf2 localization. (b) Islets were lysed and analyzed by Western blot after 48 h culture with or without oltipraz. Representative blots for Nrf2 protein (95 kDa) and the housekeeping protein PCNA (36 kDa) are shown. Coculture with oltipraz did not significantly elevate Nrf2 protein.

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