Renalase Attenuates Mouse Fatty Liver Ischemia/Reperfusion Injury through Mitigating Oxidative Stress and Mitochondrial Damage via Activating SIRT1

Figure 1

RNLS is downregulated in HFD-induced fatty livers and OA-induced steatotic HepG2 cells. (a) Gross liver from C57 mice fed a CD or a HFD for 20 weeks. Representative liver sections stained with HE (b) or Oil Red O (c) are shown. Original magnification, ×100 and ×400. (d) Immunohistochemical staining of RNLS in liver tissues from CD and HFD C57 mice. Original magnification, ×100 and ×400. The brown stained ratio in each group was analyzed. RNLS protein levels and relative mRNA expression in liver tissues and in the serum from CD and HFD C57 mice were evaluated by western blotting, RT-qPCR, and ELISA (e, f). (g) Oil Red O staining of HepG2 cells treated with different concentrations of OA. Original magnification, ×200. (h) TG concentration in HepG2 cells treated with different concentrations of OA. (i) Cell viability of HepG2 cells treated with different concentrations of OA. RNLS protein levels and relative mRNA expression in HepG2 cells treated with or without OA were evaluated by immunofluorescence (j), western blotting, and RT-qPCR (k). Original magnification, ×200. , . Data are plotted as the from three independent experiments. Abbreviation: CD: control diet; HFD: high-fat diet; OA: oleic acid; TG: triglyceride.

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