Renalase Attenuates Mouse Fatty Liver Ischemia/Reperfusion Injury through Mitigating Oxidative Stress and Mitochondrial Damage via Activating SIRT1

Figure 3

RNLS activates SIRT1 through increasing the NAD⁺ content to protect against liver IR injury. (a) RNLS, SIRT1 protein levels, and activity of SIRT1 in liver tissues and HepG2 cells. ^#ac-p53 normalized to p53; . (b) Expression and activity of SIRT1 in HepG2 cells with RNLS knockdown or RNLS administration. vs. si-NC group. (c) Cell viability of HepG2 cells pretreated with NAD⁺ at different concentrations followed by 300 μM t-BHP treatment. (d) Cell viability of HepG2 cells pretreated with NAD⁺ followed by 300 μM t-BHP treatment with or without EX527 administration. (e) Bax, Bcl-2, and SIRT1 protein levels and activity of SIRT1 in HepG2 cells pretreated with NAD⁺ followed by 300 μM t-BHP treatment with or without EX527 administration. ^#ac-p53 normalized to p53; , vs. NC group; ^$$ vs. t-BHP group, ^&& vs. t-BHP+NAD⁺ group. Data are plotted as the from three independent experiments.

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