Renalase Attenuates Mouse Fatty Liver Ischemia/Reperfusion Injury through Mitigating Oxidative Stress and Mitochondrial Damage via Activating SIRT1

Figure 5

RNLS protects against IR-induced hepatic necrosis in vivo. (a) The livers of the IR and IR+RNLS groups from CD and HFD mice were subjected to histological evaluation by HE staining. Original magnification, ×100 and ×400. (b) TUNEL staining of the livers of the sham, IR, and IR+RNLS groups from CD and HFD mice. Original magnification, ×100. (c) The expression levels of liver apoptosis related proteins were evaluated by western blotting. (d–f) Liver enzymes ALT, AST, and LDH were determined in the serum of sham, IR, and IR+RNLS mice from both CD and HFD groups. vs. sham group, ^#, ^#, and ^## vs. IR group; ^&, ^&& vs. CD group. Data are plotted as the from three independent experiments. Box plots: horizontal line within the box represents the median value, the upper and the lower limits of box indicate the 25th and 75th percentiles, and the capped vertical lines represent the minimum and maximum values. Abbreviation: TUNEL: terminal deoxynucleotidyl transferase dUTP nick end labeling; DAPI: 4,6-diamidino-2-phenylindole.

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