Research Article
Renalase Attenuates Mouse Fatty Liver Ischemia/Reperfusion Injury through Mitigating Oxidative Stress and Mitochondrial Damage via Activating SIRT1
Figure 7
RNLS suppresses IR- and t-BHP-induced oxidative stress in vivo and in vitro. (a–c) MDA and GSH levels and SOD activity in the liver tissues of sham, IR, and IR+RNLS from both CD and HFD mice. (d, e) Intracellular ROS was determined by DCFH-DA staining using fluorescence microscopy and flow cytometry. (f) Mitochondrial ROS was detected by MitoSOX red mitochondrial superoxide indicator using fluorescence microscopy. Original magnification, ×200. vs. sham or NC group; #, ## vs. IR or t-BHP; &, && vs. CD or Ctrl group. Data are plotted as the from three independent experiments. Abbreviation: MDA: malondialdehyde; GSH: glutathione; SOD: superoxide dismutase; DCFH-DA: 2,7-dichlorofluorescin diacetate.
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