Renalase Attenuates Mouse Fatty Liver Ischemia/Reperfusion Injury through Mitigating Oxidative Stress and Mitochondrial Damage via Activating SIRT1

Figure 8

RNLS alleviates the IR- and t-BHP-induced mitochondrial dysfunction. Mitochondrial membrane potential (ΔΨm) was determined by JC-1 staining using fluorescence microscopy (a) and flow cytometry (b). Liver ATP content (c) and mtDNA copy numbers (d). Representative images showing mitochondrial morphology visualized by TOM20 staining (red) using confocal microscopy; DAPI-stained nuclei were blue (e). Original magnification, ×600. The levels of mitochondrial Drp1 in each group were determined by western blotting (f). vs. NC or sham group; ^#, ^## vs. t-BHP or IR group; ^&, ^&& vs. Ctrl or CD group. Data are plotted as the from three independent experiments.

(a)

(b)

(c)

(d)

(e)

(f)