Research Article
Berberine Alleviates Amyloid β-Induced Mitochondrial Dysfunction and Synaptic Loss
Figure 3
Effect of berberine on Aβ1-42-induced reductions in axonal mitochondrial morphology. (a) Axonal mitochondrial index (number of mitochondria per 10 μm of axon) after vehicle, oligomeric Aβ1–42 (0.5 μM), or oligomeric Aβ1-42+berberine (1.0 μM) treatment for 24 h. (b) Representative images of mitochondrial distribution for the indicated treatment. Double immunostaining with MitoTracker Red (red) and Tau (green) are shown. Scale . (c) Average lengths of axonal mitochondria after vehicle, oligomeric Aβ1–42 (0.5 μM), or oligomeric Aβ1-42+berberine (1.0 μM) treatment. (d) Distribution of axonal mitochondrial lengths. (e) Cumulative distribution data for the mitochondrial lengths in panel (d) , vs. the vehicle-treated group, vs. the Aβ-treated group. Values are expressed as the error of the mean of 4 independent experiments; 8 axons per group.
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