Restoration of Autophagic Flux Rescues Oxidative Damage and Mitochondrial Dysfunction to Protect against Intervertebral Disc Degeneration

Figure 3

CUR treatment alleviates TBHP-induced degradation of the ECM in the human NP cells. (a–f) The mRNA expression levels of type II collagen, aggrecan, MMP-3, MMP-13, ADAMTS-4, and ADAMTS-5 in the human NP cells were measured by qRT-PCR. (g–m) The protein levels of type II collagen, aggrecan, MMP-3, MMP-13, ADAMTS-4, and ADAMTS-5 in the human NP cells were measured by western blotting. (n–q) Immunofluorescence staining of type II collagen and MMP-13 in the human NP cells. Scale bar: 20 μm. GAPDH was used as an internal control. Data are represented as the . , , .

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