Restoration of Autophagic Flux Rescues Oxidative Damage and Mitochondrial Dysfunction to Protect against Intervertebral Disc Degeneration

Figure 4

CUR treatment alleviates TBHP-mediated oxidative stress and mitochondrial dysfunction in the human NP cells. (a, b) The ROS levels in the human NP cells were detected using the fluorescent probe DHE and measured by flow cytometry. (c, d) Intracellular MDA levels and SOD activity in the human NP cells. (e, f) Mitochondrial membrane potential was detected by JC-1 staining and measured by flow cytometry. (g) An assessment of mPTP opening in the human NP cells. (h) Intracellular ATP levels in the human NP cells. Data are represented as the . , , .

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