Restoration of Autophagic Flux Rescues Oxidative Damage and Mitochondrial Dysfunction to Protect against Intervertebral Disc Degeneration

Figure 5

CUR treatment induces autophagy and promotes autophagic flux in the human NP cells. (a–d) The protein levels of LC3, Beclin-1, and p62 in the human NP cells were measured by western blotting. The human NP cells were treated with different concentrations of CUR (0, 5, 10, 15, 20, and 25 μM) for 24 h or treated with 25 μM CUR for different times (0, 6, 12, 18, 24, and 30 h). (e–g) The protein levels of LC3 and p62 in the human NP cells that were pretreated with or without CQ (10 μM) for 2 h and then treated with different concentrations of CUR for 24 h. (h, i) Immunofluorescence staining of LC3 in the human NP cells. Scale bar: 20 μm. GAPDH was used as an internal control. Data are represented as the . , , .

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