Restoration of Autophagic Flux Rescues Oxidative Damage and Mitochondrial Dysfunction to Protect against Intervertebral Disc Degeneration

Figure 7

CUR treatment restores defective autophagic flux in the human NP cells subjected to TBHP. (a–d) The levels of LC3 and p62 proteins in the human NP cells were measured by western blotting. (e, f) Representative images of the human NP cells expressing mRFP-GFP-LC3 were obtained by confocal microscopy. Scale bar: 10 μm. (g, h) The colocalization of LC3 and LAMP1 was examined by confocal microscopy. Scale bar: 10 μm. (i, j) LAMP2 protein level in the human NP cells was evaluated by western blotting. (k, l) The activity of CTSB and CTSD in the human NP cells. (m, n) Lysosomal pH of human NP cells was determined by LysoSensor Green DND-189 staining. Scale bar: 20 μm. GAPDH was used as an internal control. Data are represented as the . , , .

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