Multiorgan Development of Oxidative and Nitrosative Stress in LPS-Induced Endotoxemia in C57Bl/6 Mice: DHE-Based In Vivo Approach

Figure 1

Monomeric DHE oxidation products formed in vivo in the liver (a, d, and g), lung (b, e, and h), and kidney (c, f, and i) tissues in a murine model of endotoxemia, 6 and 12 hours after LPS challenge. Using HPLC-Fl detection, the superoxide-specific 2-OH-E+ and nonspecific E+ monomeric products of DHE oxidation were measured according to the details presented in Materials and Methods. Additionally, the ratio of 2-OH-E+/E+ was calculated for each sample. Data are presented as or , and statistical significance was tested using one-way ANOVA or the nonparametric Kruskal-Wallis ANOVA, depending on the distribution and homoscedasticity of data. values <0.05 were considered significant, with <0.05 and <0.01 versus the control group and ○○ <0.01 versus the LPS 6 h group.

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