Multiorgan Development of Oxidative and Nitrosative Stress in LPS-Induced Endotoxemia in C57Bl/6 Mice: DHE-Based In Vivo Approach

Figure 2

Superoxide-specific and unspecific oxidation of DHE accumulated in the liver and lung tissue in a murine model of endotoxemia, 6 and 12 hours after LPS challenge. Ratios of 2-OH-E⁺/DHE accumulated in the liver and lung tissues suggest that the liver is under increased superoxide-specific oxidant stress (a), as opposed to the lung (b). Moreover, the ratio of E⁺/DHE also suggests increased superoxide-independent oxidative stress in the liver (c), which is not the case in the lung (d). Data are presented as , and statistical significance was tested using nonparametric Kruskal-Wallis ANOVA. values <0.05 were considered significant, with <0.05 and <0.01 versus the control group, and <0.01 versus the LPS 6 h group.

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